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ELISA kit

Metronidazole ELISA Kit

1.  Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density(OD) value of the sample has a negative correlation with Metronidazole concentration in the sample. This value is compared to the standard curve and the content of the corresponding Metronidazole is subsequently obtained. 

2.  Technical specifications 
Sensitivity : 1 ppb     Detect time: Approx. 75 min
Detection limit:
Tissue, liver, intestine,shrimp,fish,honey .......... 3 ppb
Milk ................................................................ 40 ppb

Recovery rate:
Tissue, liver, honey, intestine,shrimp,fish,milk ....... 80 ±10%
Milk .................................................................... 85 ±10%

Cross-reaction rate:
   Metronidazole.......................100 %

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