This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density(OD) value of the sample has a negative correlation with Metronidazole concentration in the sample. This value is compared to the standard curve and the content of the corresponding Metronidazole is subsequently obtained.