Zilpaterol ELISA Kit

   1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of zilpaterol in the pork , liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The zilpaterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-zilpaterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the zilpaterol in it. This value is compared to the standard curve and thezilpaterol residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.20ppb

Detection limit

Tissue....................................0.2 ppb

Urine, serum........................0.5 ppb

Feed.......................................1 ppb

Recovery rate

Urine, serum........................90±10%

Tissue.....................................80±10%

Feed........................................80±15%

Cross-reaction rate

Zilpaterol ...............................100%

Clenbuterol ...........................<1%

Salbutamol.............................<1%

Brombuterol...........................<1%