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1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of zilpaterol in the pork , liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The zilpaterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-zilpaterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the zilpaterol in it. This value is compared to the standard curve and thezilpaterol residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.20ppb
Detection limit
Tissue....................................0.2 ppb
Urine, serum........................0.5 ppb
Feed.......................................1 ppb
Recovery rate
Urine, serum........................90±10%
Tissue.....................................80±10%
Feed........................................80±15%
Cross-reaction rate
Zilpaterol ...............................100%
Clenbuterol ...........................<1%
Salbutamol.............................<1%
Brombuterol...........................<1%